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KMID : 1094720200250010022
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Biotechnology and Bioprocess Engineering
2020 Volume.25 No. 1 p.22 ~ p.28
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Inhibition of 3T3-L1 Adipocyte Differentiation by D-allulose
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Moon Seo-Hyun
Kim Yang-Hee
Choi Kyung-Oh
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Abstract
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Obesity is a serious problem in modern society and its prevalence continues to increase worldwide, resulting in metabolic disorder related diseases. D-allulose, a sugar substitute, boasts a near-zero calorie value and regulates lipid accumulation. However, the molecular mechanism of D-allulose at the cellular level has not been fully elucidated. In this study, we investigated the effect of D-allulose on 3T3-L1 adipocyte differentiation. D-allulose inhibits differentiation of 3T3-L1 preadipocytes into mature adipocytes, as examined by Oil Red O staining. The mRNA levels of genes involved in lipogenesis, including fatty acid synthase (FAS) and adipocyte fatty acid-binding protein (aP2), were significantly decreased and intracellular triglyceride (TG) content was markedly reduced with D-allulose treatment. We also monitored the activity of major adipogenic transcription factors?CREB, SREBP-1c, and PPARψ?using 3T3-L1 reporter cell lines that were constructed to secrete Gaussia luciferase upon binding of a transcription factor to its DNA binding element. Collectively, D-allulose suppresses adipocyte differentiation and lipid accumulation through regulating adipogenic transcription factors and may have anti-obesity potential.
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KEYWORD
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D-allulose, adipocyte differentiation, obesity, lipid
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